BARNACLE SETTLEMENT ASSAYS
Barnacle cyprid assay. The barnacle, Balanus amphitrite Darwin, is the most ubiquitous hard fouling organism found in all marine ecosystems, particularly in ports visited by commercial shipping. The standard assay to measure the biological potential of any antifouling compound against B. amphitrite utilizes the method developed initially at Duke University Marine Laboratory (North Carolina, USA) and now in routine use at SHMRC research facility in Tuticorin, India. Briefly, the barnacle adults are cultured in the laboratory and allowed to spawn naturally. The larvae are harvested and grown in artificial culture systems until they reach the cyprid stage at which time the larvae become competent to attach to surfaces. Once attached, the cyprids transform into a pinhead barnacle, thus becoming permanently attached to the surface
This assay accomplishes the following objectives:
1.1.4a. Comparison of the EC50 against SeaNine 211, Arch’s Zinc and Copper Omadine, Frescalin, and other algaecides and anti-barnacle agents.
Cyprid Settlement Assay on Coated Dishes